Transdisciplinary Science: Mapping the Future of Research pp 263-280
Editors: Dr Mukul Kumar Baruah, Dr Rahul Kanti Nath & Dr Joyobrato Nath (2025)
ISBN: 97978-93-94174-62-7
doi : https://doi.org/10.20546/978-93-94174-62-7_22
Chapter 22
CRISPR-Cas9: A Revolutionary Tool in Plant Science
Priyanka Das*
Department of Education, Assam University Silchar, Assam-788011, India
Abstract
The clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) system is a bacterial defence mechanism system against phage infection and plasmid transfer in nature. This mechanism has been repurposed as a powerful RNA-guided exogeneous DNA targeting platform for genome editing, transcriptional perturbation, epigenetic modulation, and genome imaging. Over the subsequent years, it has received extensive attention owing to its easy manipulation, high efficiency, and wide application in gene editing and Transcriptional regulation in plants. In this chapter an attempt has been made to understand the mechanism of this revolutionized phenomena and how this technology changed the genetic variation landscape of gene editing in plant science.
Keywords
CRISPR-Cas9, Genome Editing, Plant Science, Genetic Variation, Double-Strand Breaks (DSBs), Non-Homologous End Joining (NHEJ), Homology Directed Repair (HDR), sgRNA, Cas9 Nuclease, Protospacer Adjacent Motif (PAM), Agrobacterium Mediated Transformation, Particle Bombardment, Protoplast Transfection, Ribonucleoprotein (RNP), Crop Improvement, Disease Resistance, Ethical Considerations
Cite this Chapter: Priyanka Das. 2025. CRISPR-Cas9: A Revolutionary Tool in Plant Science. In: Mukul Kumar Baruah, Rahul Kanti Nath and Joyobrato Nath (Eds.), Transdisciplinary Science: Mapping the Future of Research. Excellent Publishers, India. pp. 263-280. doi: https://doi.org/10.20546/978-93-94174-62-7_22
