Editors: Dr Mukul Kumar Baruah, Dr Rahul Kanti Nath & Dr Joyobrato Nath (2025)

ISBN: 97978-93-94174-62-7

Qualitative and Quantitative Analysis of Phytoconstituents and Assessment of Antibacterial Activity of Seed Extracts of Coixlacryma-jobi

Department of Botany, St. Joseph University, Nagaland, 797115, India

Coixlacryma-jobi L, a valuable grain from the Poaceae family, is widely cultivated for its dietary and medicinal value. This study investigates the phytochemical composition and antibacterial potential of Coixlacryma-jobi L., seed extracts obtained using methanol, acetone and chloroform. Phytochemical screening revealed the presence of multiple bioactive compounds, including alkaloids, phenols, saponins carbohydrates, flavonoids, proteins, tannins and anthraquinones in all extracts while glycosides and coumarins were found to be solvent-specific. Quantitative analysis showed that the methanolic extract yielded the highest total phenolic content (112.93mg GAE/ g) and total flavonoid content (39.88mg CE/g) followed by acetonic and chloroformic extracts. Antibacterial activity assessed against Staphylococcus aureus and Escherichia coli, exhibited inhibition by methanolic and acetonic extracts, with zones measuring up to 25mm. The antibacterial activity of extracts varied with the bacteria, showing greater effectiveness against Staphylococcus aureus. These findings highlight the importance of Coixlacryma-jobi L. seed as a storehouse of phytochemicals and natural antibacterial agent, particularly when extracted with polar solvent like methanol.

*Corresponding author; e-mail: ngangomnganbi@gmail.com

Cite this Chapter: Temsurenla Jamir and Keneisenuo. 2025. Qualitative and Quantitative Analysis of Phytoconstituents and Assessment of Antibacterial Activity of Seed Extracts of Coixlacryma-jobi. In: Mukul Kumar Baruah, Rahul Kanti Nath and Joyobrato Nath (Eds.), Transdisciplinary Science: Mapping the Future of Research. Excellent Publishers, India. pp. 250-262. doi: https://doi.org/10.20546/978-93-94174-62-7_21